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ad5 cmv 3flag egfp genechem n a antibodies  (Genechem)

 
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    Genechem ad5 cmv 3flag egfp genechem n a antibodies
    Ad5 Cmv 3flag Egfp Genechem N A Antibodies, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ad5+cmv+3flag+egfp+genechem+n+a+antibodies/aav9+carrying+cdna+usp13/pm40921878-158-8-9
    Average 86 stars, based on 1 article reviews
    ad5 cmv 3flag egfp genechem n a antibodies - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Sequencing:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Polymerase Chain Reaction:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Modification:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Transfection:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Imaging:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Lysis:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Western Blot:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Hydroxyproline Assay:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Staining:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Recombinant:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Bicinchoninic Acid Protein Assay:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Isolation:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Real-time Polymerase Chain Reaction:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..

    Software:

    Article Title: TET3 is a regulator and can be targeted for the intervention of myocardial fibrosis.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Col1a2-CreERT2 Jackson Lab #029567 Postn-CreERT2 Jackson Lab #029645 Tet3f/f Prof. Guoliang Xu N/A Piezo2f/f Cyagen #19559 Recombinant DNA Ad5-CMV-Cre-3FLAG-EGFP Genechem N/A 8 EMBO Molecular Medicine © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2025 from IP 171.247.212.166. .. Reagent/resource Reference or source Identifier or catalog number Ad5-CMV-3FLAG-EGFP Genechem N/A Antibodies This study Appendix Table S2 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 siRNA This study Appendix Table S1 Chemicals, enzymes, and other reagents Human cardiac fibroblasts Lifeline #FC-0060 Dulbecco’s modified Eagle’s medium (DMEM) Gibco #11965092 Fetal bovine serum (FBS) Gibco #10270-106 Opti-MEM Gibco #31985070 Trypsin(0.25%) Gibco #25200072 LipofectamineTM RNAiMAX Transfection reagent Invitrogen #13778150 Click-iTTM Plus EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 594 dye Invitrogen #C10639 Bobcat339 hydrochloride Selleck #S6682 D-GsMTx4 TFA Targetmol #T37697L Collagen Type I Rat Tail Corning #354236 Corning 35 mm TC-treated Culture Dish Corning #430165 Corning 60-mm TC-treated culture dish Corning #430166 Corning 100-mm TC-treated culture dish Corning #430167 Tissue culture plate-six well Jetbiofil #TCP011006 Tissue culture plate-12 well Jetbiofil #TCP011012 Tissue culture plate-48 well Jetbiofil #TCP011024 Cell lysis buffer for Western and IP Beyotime #P0013 Penicillin-Streptomycin solution Beyotime #C0222 Hydroxyproline assay kit Solarbio #BC0250 Masson’s Trichrome Stain Kit Solarbio #G1346 Picrosirius Red Stain Kit Solarbio #G1472 Recombinant human TGF-beta 1 protein R&D #7754-BH Goat anti-rabbit IgG (H+ L) crossadsorbed secondary antibody, Alexa FluorTM 594 Thermo Fsher #A-11012 Thermo Scientific PageRuler Plus Thermo Fisher #26620 Pierce BCA Protein Assay Kit Thermo Fisher #23225 Phenylmethylsulfonyl fluoride(PMSF) Thermo Fisher #36978 Tamxifen Sigma #T5648 DMSO Sigma #D8418 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro Vazyme #TD904-01 VAHTS DNA Clean Beads Vazyme #N411 TruePrep Index Kit V2 for Illumina Vazyme #TD202 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme #TD711 Reagent/resource Reference or source Identifier or catalog number FastPure Cell/Tissue Total RNA Isolation Kit Vazyme #RC101-01 HiScript II Q RT SuperMix for qPCR Vazyme #R222-01 ChamQ SYBR Color qPCR Master Mix Vazyme #Q411-02 Software GraphPad Prism9 GraphPad Software, Inc RRID:SCR_002798 ImageJ-Fiji https:// imagej.net/ software/fiji/ RRID:SCR_002285 Adobe Illustrator Adobe Systems RRID:SCR_010279 ..



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